ABSTRACT Curcuma longa are traditionally used for the treatment of ulcers, hepatic disorder, wound healing and boost glucose control. This study assessed the phytochemical compositions (quantitative and qualitative), antibacterial activities of C. longa crude extracts, synthesis and characterisation of silver nanoparticles, antibacterial activity of extract-mediated silver nanoparticles and wound activity of EeaAgNPs. The antibacterial activity of crude extracts and extract mediated silver nanoparticles (AgNPs) of C. longa rhizomes were evaluated against isolates from patients with diabetic foot infection using agar well diffusion method. Quantitative determination of phytoconstituents revealed a significant amount of phytates (6577.9 mg/100 g), cyanides (2741.8 mg/100 g) and saponins (618.0 mg/100 g). Cold maceration of rhizome with 70% ethanol yielded a crude extract (E). Successive partitioning of extract E with chloroform and ethyl acetate yielded chloroform (Ec), ethyl acetate (Eea) soluble fractions, as well as aqueous residual fraction (Eaq). Qualitative screening of the extract and fractions revealed the presence of flavonoids, reducing sugars, anthraquinones, tannins and saponins. The extract and its fractions at 100 mg/ml were inactive on Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes and Staphylococcus aureus while, fraction Eea at 200mg/ml was active on P. aeruginosa, E. coli and S. pyogenes. The wavelength of EAgNPs, Ec-AgNPs, Eea-AgNPs, and Eaq-AgNPs were 405 nm, 406 nm, 409 nm and 410 nm respectively. The FTIR indicated the presence of aromatic, alkanes, alkynes, alkenes and carboxylic functional groups while the SEM micrograph of Eea-AgNPs revealed clustered rod-like morphology. The highest XRD peak was at 2ϴ (340 ). The Eea-AgNPs at 200 mg/ml was active on S. pyogenes, P. aeruginosa, E. coli and K. pneumoniae with zones of inhibition of 7 ± 1.7 mm, 10 ± 0.7 mm, 11 ± 1.1 mm and 14 ± 0.5 mm respectively. The MIC of Eea-AgNPs against test isolates was at 12.5 mg/ml. The extract was bacteriostatic on the test isolates. There was significant (P<0.05) wound closure observed in rats (groups 1 to 6) topically treated with Eea-AgNPs ointment from Day 0 (1.24 ± 0.00 mm to 1.29 ± 0.19 mm) to Day 14 (0.4 ± 0.1 mm to 0.73 ± 0.00 mm) compared to group 7 (Diabetes + Wound only) with (1.23 ± 0.00 to 1.1 ± 0.3 mm). Histology of the treated rats indicated wound healing characterized with collagens, fibroblasts, inflammatory cells, new blood vessels, granulation tissues and complete epithelialization. The application of ointment on rats produced no allergic reactions, rashes and other forms of skin irritation. These findings showed the potentials of C. longa as a safe therapeutic agent to treat and heal infected ulcer.
ABSTRACT
This research examined the impact of inventory control on the profitability of manufacturing c...
ABSTRACT
Result processing can be a continuous process of converting data (scores, grade p...
ABSTRACT
The study examined the impact of modern office machines on secretaries using Ministry of Education Accra Metrop...
Abstract
Many developing countries are competing to attract foreign direct investment with a belief that it can be a tool for poverty red...
Background Of The Study
Health services are deemed unsatisfactory and inadequate in addressing the publ...
ABSTRACT
The study delved into the effect of the use of agrochemical in rice farming using Ufuma, Orumb...
Background of the study
The issue of youth joblessness and criminal activities in rural areas has, over the...
IMPORTANCE OF STUDY
It is usually a concern over the chemical composition or contamination of foods and the effect this has on its value...
ABSTRACT
The study was to assess the gate keeping functions of the two media ownership in Nigeria (Government and Privat...
ABSTRACT
This study was on the factors associated with mass failure of students in the Accounting education in secondary...